Blar i forfatter "Popova, Daria"

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    • Advanced methods in reproductive medicine: Application of optical nanoscopy, artificial intelligence-assisted quantitative phase microscopy and mitochondrial DNA copy numbers to assess human sperm cells 

      Popova, Daria (Doctoral thesis; Doktorgradsavhandling, 2021-10-22)
      Declined fertility rate and population is a matter of serious concern, especially in the developed nations. Assisted Reproductive Technologies (ART), including in vitro fertilization (IVF), have provided great hope for infertility treatment and maintaining population growth and social structure. With the help of ART, more than 8 million babies have already been born so far. Despite the worldwide ...

    • High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition 

      Butola, Ankit; Popova, Daria; Prasad, Dilip K.; Ahmad, Azeem; Habib, Anowarul; Tinguely, Jean-Claude; Basnet, Purusotam; Acharya, Ganesh; Paramasivam, Senthilkumaran; Mehta, Dalip Singh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-04)
      Sperm cell motility and morphology observed under the bright field microscopy are the only criteria for selecting a particular sperm cell during Intracytoplasmic Sperm Injection (ICSI) procedure of Assisted Reproductive Technology (ART). Several factors such as oxidative stress, cryopreservation, heat, smoking and alcohol consumption, are negatively associated with the quality of sperm cell and ...

    • Live-cell imaging of human spermatozoa using structured illumination microscopy 

      Opstad, Ida Sundvor; Popova, Daria; Acharya, Ganesh; Basnet, Purusotam; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-05)
      Structural details of spermatozoa are interesting from the perspectives of fundamental biology and growing reproductive health problems. Studies of nanostructural details of these extremely motile cells have been limited to fixed cells, largely using electron microscopy. Here we provide the protocols for and demonstrate live-cell multi-color super-resolution imaging of human spermatozoa using ...

    • Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress condition 

      Dubey, Vishesh Kumar; Popova, Daria; Ahmad, Azeem; Acharya, Ganesh; Basnet, Purusotam; Mehta, Dalip Singh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-05)
      Semen quality assessed by sperm count and sperm cell characteristics such as morphology and motility, is considered to be the main determinant of men’s reproductive health. Therefore, sperm cell selection is vital in assisted reproductive technology (ART) used for the treatment of infertility. Conventional bright field optical microscopy is widely utilized for the imaging and selection of sperm cells ...

    • Sperm mitochondrial DNA copy numbers in normal and abnormal semen analysis: A systematic review and meta-analysis 

      Popova, Daria; Bhide, Priya; D’Antonio, Francesco; Basnet, Purusotam; Acharya, Ganesh (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-25)
      Background: Normal mature sperm have a considerably reduced number of mitochondria, which provide the energy required for progressive sperm motility. Literature suggests that disorders of sperm motility may be linked to abnormal sperm mitochondrial number and function.<p> <p>Objectives: To summarise the evidence from literature regarding the association of mitochondrial DNA copy numbers and semen ...